Movie EV1: Live cell imaging 
A. Live cell imaging of HeLa cells stably expressing PEROXO-tag (3xMyc-EGFP-PEX26) infected with mCherry-STM (MOI=50). Cells were also treated with 100 nM of LysoTracker (blue) for 15 min before imaging to label acidic vacuoles. Time-lapse imaging was performed 5 h post-infection. Images were captured approximately every minute (80 s) till 16 min. Images shown are single Z slices for the indicated time pointsscale bar:10 m.  
